Lindsey Hillesheim
lhilles@physics.umn.edu
Education
B.S., Valparaiso University (1999)
Ph.D., University of Minnesota (2005)
Research
The Mueller lab is primariliy concerned with the refinement and
extension of Fluorescence Fluctuation Spectrscopy (FFS). FFS utilizes
the
flucutations produced by fluorescently-tagged biomolecules to study
biological systems both in solution and in cells. My work focuses on
the development of dual-color Photon Counting Histogram (PCH) as a
fluctuation analysis tool. I am
particularly interested in applying dual-color PCH analysis to study
protein-protein interactions in living cells and towards that end, I
use intrisically fluorescent proteins such as EGFP and mRFP as probes
of those interactions.
I have refined both single channel and dual-color PCH models to include
non-ideal detector effects such as afterpulsing and dead-time. I am
currently looking at how Fluorescent Resonance Energy Transfer (FRET)
affects the resolvability of species in dual-color PCH analysis and how
to include such effects in the dual-color PCH.
Web Page
http://www.physics.umn.edu/directory/lhilles.html